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A recommended esterification method for gas chromatographic measurement of conjugated linoleic acid

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Journal of the American Oil Chemists' Society

Abstract

The effect of the methylation method on isomerization of conjugated linoleic acid (CLA) in gas chromatographic analysis was studied. Among methylation methods examined, the magnitude of isomerization of CLA was greatest with BF3 catalyst, followed by HCI and H2SO4 catalyst. Short-time methylation did not extensively change the CLA composition in all methods, and c,t and t,c isomers were essentially maintained, while the appearance of t,t isomers and unknown peaks was practically restricted. After 120 min of methylation, there was essentially no conversion in the H2SO4 method, in contrast to a marked change in the BF3 method. The antioxidants butylated hydroxytoluene, ascorbic acid, β-carotene, and α-tocopherol did not suppress conversion, while dimethylsulfoxide (DMSO) and dimethylformamide (DMF) attenuated the changes in CLA composition. Suppression was more effective in the H2SO4 method than in the BF3 method. Thus, methylation with H2SO4 in the presence of a proper amount of DMSO or DMF is recommended for esterification of CLA.

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Authors and Affiliations

  1. Koji Yamada, Laboratory of Food Science, Department of Food Science and Technology, Faculty of Agriculture, Kyushu University 46-09, 6-10-1 Hakozaki, Higashiku, 812-8581, Fukuoka, Japan

    Masao Yamasaki, Koji Kishihara & Koji Yamada

  2. Laboratory of Nutrition Chemistry, Department of Food Science and Technology, Faculty of Agriculture, Kyushu University 46-09, 812-8581, Fukuoka, Japan

    Ikuo Ikeda

  3. Faculty of Human Life Sciences, Prefectural University of Kumamoto, 862-8502, Kumamoto, Japan

    Michihiro Sugano

Authors
  1. Masao Yamasaki
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  2. Koji Kishihara
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  3. Ikuo Ikeda
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  4. Michihiro Sugano
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  5. Koji Yamada
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Correspondence to Koji Yamada.

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Yamasaki, M., Kishihara, K., Ikeda, I. et al. A recommended esterification method for gas chromatographic measurement of conjugated linoleic acid. J Amer Oil Chem Soc 76, 933–938 (1999). https://doi.org/10.1007/s11746-999-0109-0

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  • DOI: https://doi.org/10.1007/s11746-999-0109-0

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